Journal: Immunity

Article Title: A modified vaccinia Ankara vector-based vaccine protects macaques from SARS-CoV-2 infection, immune pathology, and dysfunction in the lungs

doi: 10.1016/j.immuni.2021.02.001

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Article Snippet: SARS-CoV-2 (2019-nCoV) Spike RBD Antibody, Rabbit PAb, Antigen Affinity Purified , Sino Biologicals , Cat#40592-T62 , .

Techniques: Adsorption, Affinity Purification, Modification, Recombinant, Transfection, Peptide Microarray, Plasmid Preparation, Expressing, Software

Journal: Life Science Alliance

Article Title: Expression of TMPRSS2 is up-regulated by bacterial flagellin, LPS, and Pam3Cys in human airway cells

doi: 10.26508/lsa.202201813

Figure Lengend Snippet: (A) Calu-3 cells were treated with flagellin (200 ng/ml) for 24 h, then infected with H1N1pdm or H3N2 IAV at a low MOI and incubated for 48 h in the absence or presence of trypsin. Virus titers in supernatants were determined by the plaque assay at the indicated time points. Data shown are mean values + SD of four independent experiments. (B) Calu-3 cells stimulated with flagellin (fla) for 24 h as described above were inoculated with H1N1pdm at an MOI of 0.001 and incubated for 48 h with or without exogenous trypsin (try). Cell lysates were subjected to SDS–PAGE and Western blot analysis using H1N1-specific antibodies. β-actin served as a loading control. (C) Calu-3 cells were incubated with flagellin for 24 h and then infected with SARS-CoV-2 or SARS-CoV at a low MOI. Virus titers were determined by TCID 50 titration at indicated time points. Data are mean values + SD (n = 3). Statistics: a t test or Welch’s t test, ns P > 0.05 compared with the untreated infected control.

Article Snippet: A polyclonal rabbit serum against HA of H1N1 was obtained from Sino Biological (11085-T62).

Techniques: Infection, Incubation, Plaque Assay, SDS Page, Western Blot, Titration